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Related Experiment Video

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Microbiome Helper: a Custom and Streamlined Workflow for Microbiome Research.

André M Comeau1, Gavin M Douglas1, Morgan G I Langille1

  • 1CGEB-Integrated Microbiome Resource (IMR) and Department of Pharmacology, Dalhousie University, Halifax, Canada.

Msystems
|January 10, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces Microbiome Helper, a free resource simplifying microbiome research. It offers easy sequencing protocols and bioinformatic tools, making microbial analysis accessible for new researchers.

Keywords:
16S rRNA gene sequencingMicrobiome Helperbioinformaticsdual-indexing PCReducationmetagenomicsmicrobiomestandard operating procedurevirtual machine

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Area of Science:

  • Microbiome Research
  • Bioinformatics
  • Molecular Biology

Background:

  • Sequence-based microbiome studies (e.g., 16S rRNA sequencing, metagenomics) reveal microbial associations but involve complex, evolving protocols.
  • The complexity of library preparation and bioinformatics presents a significant barrier for researchers new to the microbiome field.

Purpose of the Study:

  • To provide a streamlined, cost-effective, and accessible resource for conducting microbiome experiments from sample processing to data analysis.
  • To lower the barrier to entry for new researchers in the rapidly growing microbiome field.

Main Methods:

  • Development of a simplified, custom gene sequencing protocol requiring minimal lab equipment.
  • Creation of integrated bioinformatic scripts available as a standalone installation or virtual image.
  • Compilation of step-by-step bioinformatic workflows and tutorials for user education.

Main Results:

  • A comprehensive, freely available resource (Microbiome Helper) encompassing protocols, scripts, and tutorials.
  • The resource has been tested on various sample types, ensuring reliability and broad applicability.
  • All components are accessible via the Microbiome Helper website.

Conclusions:

  • Microbiome Helper provides essential guidance and best practices for quality microbiome research.
  • This resource enables rapid and reliable microbiome analysis, allowing researchers to focus on experimental design and interpretation.
  • The platform will be continuously updated with new protocols and training materials to support the evolving field.