Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multidimensional scaling informed by F-statistic: Visualizing grouped microbiome data with inference.

PLoS computational biology·2026

Same author

Spatially structured bacterial interactions alter algal carbon flow to bacteria.

The ISME journal·2025

Same author

MCount: An automated colony counting tool for high-throughput microbiology.

PloS one·2025

Same author

Genome-scale resources in the infant gut symbiont Bifidobacterium breve reveal genetic determinants of colonization and host-microbe interactions.

Cell·2025

Same author

Zeta potential characterization using commercial microfluidic chips.

Lab on a chip·2023

Same author

A mutant fitness compendium in Bifidobacteria reveals molecular determinants of colonization and host-microbe interactions.

bioRxiv : the preprint server for biology·2023

Search research articles

Related Experiment Video

Updated: Mar 9, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

High efficiency hydrodynamic bacterial electrotransformation.

Paulo A Garcia¹, Zhifei Ge¹, Laura E Kelley²

¹Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA. crb@mit.edu.

|January 10, 2017

Summary

This study introduces a novel microfluidic system to enhance bacterial electrotransformation. The new method significantly boosts genetic transformation efficiency and throughput for microbes, accelerating synthetic biology applications.

More Related Videos

Electroporation of Mycobacteria

Electroporation of Mycobacteria

Published on: May 23, 2008

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

Related Experiment Videos

Last Updated: Mar 9, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Electroporation of Mycobacteria

Electroporation of Mycobacteria

Published on: May 23, 2008

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

Area of Science:

Synthetic Biology
Biotechnology
Microfluidics

Background:

Synthetic biology aims to address global challenges, but faces limitations due to low genetic transformation efficiency and throughput.
Bacterial electrotransformation is a key technique in genetic engineering, but current methods are often inefficient.

Purpose of the Study:

To develop and evaluate a novel microfluidic system for improving bacterial electrotransformation.
To increase both the efficiency and throughput of genetic transformation in microbial cells.

Main Methods:

A microfluidic device with non-uniform constrictions was designed to generate high electric fields for electroporation.
The system incorporates regions of low electric field for electrophoretic transport of nucleic acids.
Hydrodynamically controlled electric fields were utilized to provide time-dependent electrical stimulation.

Main Results:

The microfluidic system demonstrated a ~4x increase in transformation efficiency compared to traditional methods.
Throughput was enhanced by 100-1000x, indicating a significant improvement in processing capacity.
The system enables high-efficiency and high-throughput genetic transformation of microbes.

Conclusions:

The developed microfluidic system offers a substantial advancement for bacterial electrotransformation.
This technology facilitates accelerated development of genetically engineered organisms through improved transformation capabilities.
The findings pave the way for broader applications of synthetic biology.