Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging
Francesco Cutrale1,2, Vikas Trivedi1,2,3,4, Le A Trinh1,2
1Translational Imaging Center, University of Southern California, Los Angeles, California, USA.
Abstract:
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
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