Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Minimum essential structural requirements for lactam antibiotic action.

P Kovacic1, J R Ames, M D Ryan

  • 1Department of Chemistry, University of Wisconsin, Milwaukee 53201.

Free Radical Research Communications
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression and immunogenicity of secreted forms of bovine ephemeral fever virus glycoproteins applied to subunit vaccine development.

Journal of applied microbiology·2021
Same author

Theiler's murine encephalomyelitis virus contrasts with encephalomyocarditis and foot-and-mouth disease viruses in its functional utilization of the StopGo non-standard translation mechanism.

The Journal of general virology·2012
Same author

Dynamic electrochemistry: methodology and application.

Analytical chemistry·2010
Same author

Signed outside: a surface marker system for transgenic cytoplasmic proteins.

Gene therapy·2010
Same author

Creatinine clearance and blood pressure: a 34-year circadian study.

La Clinica terapeutica·2009
Same author

Circadian characteristics of serum calcium, magnesium and eight trace elements and of their metallo-moieties in urine of healthy middle-aged men.

La Clinica terapeutica·2008
Same journal

Reduction of a tetrazolium salt and superoxide generation in human tumor cells (HeLa).

Free radical research communications·1993
Same journal

Radical-induced damage to bovine serum albumin: role of the cysteine residue.

Free radical research communications·1993
Same journal

Identification of products from oxidation of uric acid induced by hydroxyl radicals.

Free radical research communications·1993
Same journal

Influence of hyperthyroidism on superoxide radical and hydrogen peroxide production by rat liver submitochondrial particles.

Free radical research communications·1993
Same journal

Spin-trapping and chemiluminescence studies of neutrophils from a Holstein-Friesian calf with bovine leukocyte adhesion deficiency.

Free radical research communications·1993
Same journal

The effect of oxygen at physiological levels on the detection of free radical intermediates by electron paramagnetic resonance.

Free radical research communications·1993
See all related articles

This study provides evidence that electron transfer (ET) is crucial for beta-lactam antibiotic activity. The formation of a conjugated iminium entity is essential for enzyme inactivation and therapeutic effect.

Area of Science:

  • Medicinal Chemistry
  • Biochemistry
  • Organic Chemistry

Background:

  • A prior mechanism proposed electron transfer (ET) as essential for beta-lactam antibiotic action.
  • This ET involves the formation of a conjugated iminium entity, linked to enzyme inactivation.

Purpose of the Study:

  • To provide additional evidence supporting the ET mechanism for beta-lactam antibiotics.
  • To investigate the role of specific functional groups in ET and antibiotic activity.

Main Methods:

  • Electrochemical reduction potentials were measured for various cephalosporins and pyrazolidinones.
  • Compounds with and without imine functionalities were compared.
  • Related agents like hydrazones, oxamazins, and lactivicin were analyzed.

Related Experiment Videos

Main Results:

  • Cephalosporins and pyrazolidinones with oximino side chains exhibited reduction potentials between -0.6 and -0.7 V.
  • These findings support the proposed ET mechanism.

Conclusions:

  • The electron transfer (ET) mechanism, involving conjugated iminium formation, is a key determinant of beta-lactam antibiotic activity.
  • The presence of specific side chain functionalities influences ET and, consequently, antibiotic efficacy.