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Updated: Mar 9, 2026
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
Jiao Yang1, Lei Zhou1, Qinglian Liu1
1Department of Physiology and Biophysics, School of Medicine, Virginia Commonwealth University, VA 23298, USA.
Abstract:
Human BiP/GRP78 is involved in the folding and assembly of proteins in the endoplasmic reticulum. The proteins for crystallization in good amount and quality are prerequisites for obtaining ideal crystals. To meet these requirements, different BiP/GRP78 constructs, competent cells, vectors, and concentrations of inducer were tested in order to obtain soluble BiP/GRP78 protein with the highest amount and best purity. The BiP-T229A-L3,4'-Smt3 fusion protein was expressed in a soluble manner and finally purified with the highest purity using size exclusion chromatography, which was suitable for further protein crystallization.
