Electrostatic layer-by-layer construction of fibrous TMV biofilms
Brylee David B Tiu1, Daniel L Kernan2, Sicily B Tiu3
1Department of Biomedical Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, OH 44106, USA and Department of Macromolecular Science and Engineering, Case Western Reserve University School of Engineering, Cleveland, OH 44106, USA. rca41@case.edu.
Nanoscale
|January 11, 2017
Summary
Researchers used charged tobacco mosaic virus (TMV) nanoparticles to create multilayered fibrous networks. These biomaterials mimic the extracellular matrix, supporting cell adhesion and forming free-standing membranes for potential drug delivery applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Regenerative Medicine
Background:
- Bottom-up assembly of nanoscale building blocks offers advantages over top-down manufacturing for complex morphologies.
- Charged nanoparticles, like tobacco mosaic virus (TMV), are promising for creating advanced materials.
- Electrostatic interactions can drive controlled self-assembly of nanomaterials.
Purpose of the Study:
- To construct multilayered fibrous networks using charged tobacco mosaic virus (TMV) nanoparticles.
- To investigate the potential of these TMV assemblies in regenerative medicine and drug delivery.
- To develop free-standing biomembranes from TMV constructs.
Main Methods:
- Utilized electrostatic layer-by-layer (LbL) deposition with anionic TMV-wt and lysine-functionalized TMV-lys nanoparticles.
- Investigated the self-assembly driven by electrostatic interactions between TMV variants.
- Assessed the ability of TMV assemblies to support NIH-3T3 fibroblast cell adhesion.
- Developed free-standing TMV scaffolds and programmed cargo-functionalized biofilms.
Main Results:
- Successfully constructed highly controlled, multilayered fibrous networks of TMV nanoparticles.
- TMV assemblies mimicked the 3D fibrous network of the extracellular matrix (ECM).
- Demonstrated support for NIH-3T3 fibroblast cell adhesion, indicating regenerative medicine potential.
- Created free-standing TMV biomembranes and cargo-functionalized biofilms for potential applications.
Conclusions:
- Electrostatic LbL deposition of charged TMV nanoparticles is an effective method for creating complex biomaterials.
- TMV-based fibrous networks show promise for tissue engineering and regenerative medicine applications.
- Programmable TMV biofilms offer potential for advanced drug delivery and vaccine technologies.
