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Chemoselective Henry Condensations Catalyzed by Artificial Carboligases
Xavier Garrabou1, Duncan Stuart Macdonald1, Donald Hilvert1
1Laboratory of Organic Chemistry, ETH Zürich, 8093, Zürich, Switzerland.
Abstract:
The promiscuity of de novo designed enzymes provides a privileged platform for diverse abiological reactions. In this work, we report the first example of a nitroolefin synthase that catalyzes the Henry condensation between aromatic aldehydes and nitromethane. Significant catalytic activity was discovered in the computationally designed and evolved carboligase RA95.5-8, and mutations around the active site were shown to improve the reaction rate, demonstrating the potential to optimize the enzyme by directed evolution. This novel nitroolefin synthase could participate in complex biological cascades, whereby the highly tunable chemoselectivity could afford useful synthetic building blocks.
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