Related Experiment Video

Updated: Mar 9, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

8.1K

ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer

Xian Hua Gao1, Juan Li, Yan Liu

  • 1Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University Department of Nephrology, Changhai Hospital, Second Military Medical University Department of Epidemiology, Second Military Medical University, Shanghai, China.

Medicine
|January 11, 2017
PubMed
Abstract

Insights

Zinc finger protein 148 (ZNF148) and TOP2A RNA molecules regulate each other via competing endogenous RNA (ceRNA) mechanisms in colorectal cancer (CRC). This interaction impacts cell proliferation, offering new insights into CRC development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Competing endogenous RNA (ceRNA) regulation is a novel mechanism where RNA molecules share target microRNAs (miRNAs).
  • This interaction allows RNA molecules to compete for binding to the same miRNA pool, influencing gene expression.

Purpose of the Study:

  • To investigate the competing endogenous RNA (ceRNA) regulatory mechanism between Zinc finger protein 148 (ZNF148) and TOP2A in colorectal cancer (CRC).
  • To determine the impact of this ceRNA interaction on CRC cell proliferation.

Main Methods:

  • Analyzed ZNF148 and TOP2A expression in 742 CRC tissues using immunohistochemistry (IHC).
  • Quantified mRNA and miRNA expression in 53 CRC tissues via reverse transcription polymerase chain reaction.
  • Employed bioinformatics, correlation analysis, RNA interference, gene overexpression, and luciferase assays to elucidate ceRNA mechanisms.

Main Results:

  • ZNF148 and TOP2A protein and mRNA levels showed positive correlations in CRC tissues.
  • Bioinformatics identified 13 common target miRNAs for ZNF148 and TOP2A, including miR101, miR144, miR335, and miR365.
  • ZNF148 and TOP2A were found to regulate each other through a ceRNA mechanism involving their 3'UTRs, impacting CRC cell proliferation.

Conclusions:

  • ZNF148 and TOP2A engage in a mutual ceRNA regulatory mechanism within colorectal cancer.
  • This interaction significantly influences colorectal cancer cell proliferation, highlighting a novel regulatory pathway in CRC development.

More Related Videos

The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.1K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
10.6K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
969
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K