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FRET Imaging in Three-dimensional Hydrogels
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Temperature Mapping in Hydrogel Matrices Using Unmodified Digital Camera.

Ghinwa H Darwish1, Hassan H Fakih1, Pierre Karam1

  • 1Department of Chemistry, American University of Beirut , P.O. Box 11-0236, Beirut 1107 2020, Lebanon.

The Journal of Physical Chemistry. B
|January 11, 2017
PubMed
Summary
This summary is machine-generated.

A novel fluorescent nanothermometer maps temperature changes in hydrogels using a DSLR camera. This noninvasive method, based on polymer complexation, offers reversible and sensitive thermal mapping for soft materials.

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Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Optical Sensing

Background:

  • Hydrogels are versatile soft materials with applications in various fields.
  • Accurate mapping of thermal fluctuations within hydrogels is crucial for understanding their behavior.
  • Existing methods for thermal mapping can be invasive or lack sufficient resolution.

Purpose of the Study:

  • To develop a simple, noninvasive, and broadly applicable fluorescent method for mapping thermal fluctuations in hydrogel matrices.
  • To utilize a commercially available digital single-lens reflex (DSLR) camera for this thermal mapping.
  • To establish a nanothermometer based on polymer complexation for temperature sensing.

Main Methods:

  • Complexation of poly(phenylene ethynylene) carboxylate with polyvinylpyrrolidone within a gel matrix.
  • Utilizing the fluorescent ratiometric response of the polymer complex to temperature changes.
  • Capturing fluorescent signal changes with a DSLR camera and analyzing RGB components using Matlab.
  • Correlating green and blue intensity channels with hydrogel temperature.

Main Results:

  • Demonstrated a reversible fluorescent ratiometric response to temperature changes in hydrogels.
  • Achieved maximum relative sensitivities of 2.0% and 1.9% at 45.0 °C for agarose and agar, respectively.
  • Observed no hysteresis when cycling samples between 20 and 40 °C.
  • Established a linear correlation between hydrogel temperature and green/blue RGB intensity channels.

Conclusions:

  • The developed fluorescent nanothermometer provides a simple and noninvasive method for mapping thermal fluctuations in hydrogels.
  • The method is generally applicable and utilizes readily available DSLR cameras for data acquisition.
  • This technique has potential for correlating thermal mapping with chemical or physical processes in soft gel matrices.