Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.8K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.8K
The DNA Replication Fork01:02

The DNA Replication Fork

42.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
42.1K
The DNA Replication Fork01:02

The DNA Replication Fork

19.0K
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same author

An orally active dual CBP/p300 degrader targets core dependencies of multiple myeloma.

Cell reports·2026
Same author

ATM counteracts chromatin-bound cGAS during DNA replication.

Nature cell biology·2026
Same author

BRCA1-A and LIG4 complexes mediate ecDNA biogenesis and cancer drug resistance.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Targeted therapy-induced chromosomal instability dictates mitotic dependency on Aurora Kinase A.

bioRxiv : the preprint server for biology·2026
Same author

ATR Safeguards Epithelial-to-Mesenchymal Transition by Countering R-loops and Enabling Transcription Reprogramming.

The Journal of clinical investigation·2026
Same journal

A global response contributes to tissue size robustness upon local induction of apoptosis.

Current biology : CB·2026
Same journal

Prebilaterian origin of monoaminergic signaling.

Current biology : CB·2026
Same journal

CLASP-dependent microtubule stabilization generates microtubule-based protrusive forces during Drosophila epithelial morphogenesis.

Current biology : CB·2026
Same journal

Pigeons make slow, divergent eye movements during flight and large, convergent eye movements when landing.

Current biology : CB·2026
Same journal

Temperature signals drive grass secondary cell wall thickening.

Current biology : CB·2026
Same journal

Neuronal RNAi and oxygen-sensing circuit shape germline resilience to heat stress.

Current biology : CB·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

17.2K

DNA Replication Checkpoint: New ATR Activator Identified.

Lee Zou1

  • 1MGH Cancer Center & Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

Current Biology : CB
|January 11, 2017
PubMed
Summary
This summary is machine-generated.

The protein ETAA1 activates the ATR kinase, a key player in DNA replication stress responses. These findings reveal how the ATR pathway is regulated during cellular stress.

More Related Videos

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K
Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K

Related Experiment Videos

Last Updated: Mar 9, 2026

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

17.2K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K
Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Genetics

Background:

  • The ATR kinase pathway is crucial for maintaining genomic stability by responding to DNA replication stress.
  • Understanding the upstream activators of ATR is essential for comprehending cellular responses to DNA damage.

Purpose of the Study:

  • To identify and characterize novel activators of the ATR kinase pathway.
  • To elucidate the role of ETAA1 in the human cellular response to replication stress.

Main Methods:

  • Investigated the interaction between ETAA1 and ATR in human cell lines.
  • Utilized biochemical assays and genetic approaches to assess ETAA1's function in ATR activation.

Main Results:

  • Demonstrated that ETAA1 is a significant activator of ATR kinase in human cells.
  • Showcased ETAA1's role in the signaling cascade initiated by replication stress.

Conclusions:

  • ETAA1 is a critical component of the ATR pathway, functioning as an upstream activator.
  • These findings provide new insights into the mechanisms governing replication stress responses and ATR pathway regulation.