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Analytical performances of Optilite® turbidimeter (The Binding Site): a new dedicated analyser for specific proteins
Pascale Ghillani1, Laurent Dufat1, Delphine Sterlin1
1Laboratoire d'immunochimie et auto-immunité, Département d'immunologie, Groupe Hospitalier Pitié-Salpêtrière-Charles Foix, APHP, France.
We checked analytical performances of Optilite® analyser for immunoglobulins G, A, M, subclasses of IgG, free light chains of Ig (Freelite®) and complement's fractions using Binding Site reagents. CVs for repeteability and reproducibility showed very good results, respectively <3% and <10% for all tested parameters, in agreement with Ricos and SFBC recommendations. Comparisons with results obtained on BN™II (Siemens) or SPAPLUS ® (Binding site) analysers showed a good agreement (>83%) according to Bland and Altman analysis. Sample throughput with either a batch of Freelite® only or Freelite® and immunoglobulins showed a gain of total realisation time on Optilite® versus BN™II. Optilite® analyser performed automatic dilutions until result and antigen excess determination for parameters as Freelite® or IgG4. In terms of practicability, traceability and maintenance, Optilite® turbidimeter alone or connected to LIS via DataSite software is well adapted to specialized laboratory for proteins determinations.
We checked analytical performances of Optilite® analyser for immunoglobulins G, A, M, subclasses of IgG, free light chains of Ig (Freelite®) and complement's fractions using Binding Site reagents. CVs for repeteability and reproducibility showed very good results, respectively <3% and <10% for all tested parameters, in agreement with Ricos and SFBC recommendations. Comparisons with results obtained on BN™II (Siemens) or SPAPLUS ® (Binding site) analysers showed a good agreement (>83%) according to Bland and Altman analysis. Sample throughput with either a batch of Freelite® only or Freelite® and immunoglobulins showed a gain of total realisation time on Optilite® versus BN™II. Optilite® analyser performed automatic dilutions until result and antigen excess determination for parameters as Freelite® or IgG4. In terms of practicability, traceability and maintenance, Optilite® turbidimeter alone or connected to LIS via DataSite software is well adapted to specialized laboratory for proteins determinations.
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