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Related Experiment Videos

A simple field test for identification of night-blindness.

P Patel, T Gopaldas, I Pant

    Indian Journal of Pediatrics
    |March 1, 1989
    PubMed
    Summary

    A new field test accurately measures dark adaptation time. Night-blind individuals showed significantly longer adaptation times compared to controls, suggesting a link to vitamin A levels.

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    Area of Science:

    • Ophthalmology
    • Nutritional Science

    Background:

    • Dark adaptation is crucial for vision in low light.
    • Night blindness (NB) can impair daily activities.
    • Vitamin A deficiency is a known cause of NB.

    Purpose of the Study:

    • To develop and validate a simple, replicable field test for measuring dark adaptation time.
    • To compare dark adaptation times in individuals with and without night blindness.
    • To explore the correlation between serum vitamin A levels and dark adaptation time.

    Main Methods:

    • A novel field test utilizing a darkened room, controlled light flashes, and a stopwatch was employed.
    • The test was administered to three groups: night-blind children (NB), non-night-blind controls (NNB), and a healthy high-income group (PC).
    • Serum vitamin A levels were measured and correlated with the measured dark adaptation times.

    Main Results:

    • The night-blind group (NB) exhibited significantly longer mean dark adaptation times (14.9 seconds) compared to both the non-night-blind controls (NNB, 11.9 seconds) and the healthy high-income group (PC, 11.3 seconds).
    • Mean serum vitamin A levels differed across groups, with the NB group showing the lowest levels (15.8 µg/dL) compared to NNB (17.5 µg/dL) and PC (29.5 µg/dL).
    • A correlation was observed between serum vitamin A levels and dark adaptation times across all groups.

    Conclusions:

    • The developed field test is a reliable method for assessing dark adaptation.
    • Impaired dark adaptation is significantly associated with night blindness.
    • Serum vitamin A levels appear to play a role in dark adaptation efficiency.

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