Implementation of a novel ultra fast metabolic stability analysis method using exact mass TOF-MS
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Updated: Mar 9, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Joseph D Manna1, Samantha J Richardson1, Mehran F Moghaddam1
1Department of Drug Metabolism & Pharmacokinetics, Celgene Corporation, 10300 Campus Point Dr., San Diego, CA 92121, USA.
Aim:
Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. Materials and methods/results: An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing. We can analyze and process 96 compounds across two species in quadruplicate in a 24-h period with no method development.
Conclusion:
An advanced bioanalytical workflow has increased our capacity threefold and reduced our instrument/processing needs threefold.
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