Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sinogram-based flow estimation in computed tomography using a physics-informed neural network: Impact of gantry rotation speed, X-ray fluence and pulsed acquisition on accuracy.

Medical physics·2026
Same author

Mechanically Programmable DNA Hydrogel Microparticles for 3D Cellular Systems.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Neutrophils exhibit distinct migration phenotypes that are modulated by transendothelial migration.

Communications biology·2026
Same author

Guidance of cellular nematic elastomers into shape-programmable living surfaces.

Science (New York, N.Y.)·2026
Same author

Left atrial flow and thrombosis risk from 4D CT contrast dynamics by physics-informed neural network and indicator dilution theory.

bioRxiv : the preprint server for biology·2026
Same author

Physiomimetic culture bias durotaxis toward soft environments.

bioRxiv : the preprint server for biology·2026
Same journal

Application of ephrin-B2 loaded glycol chitosan-silk fibroin hydrogel in the treatment of diabetic refractory wounds.

Scientific reports·2026
Same journal

International expert Delphi consensus on thromboprophylaxis in metabolic and bariatric surgery.

Scientific reports·2026
Same journal

Assessing the cross-region knowledge transfer capability of selected deep learning building vectorization methods in the context of available training datasets.

Scientific reports·2026
Same journal

Feasibility and preliminary effects of outdoor versus indoor cognitive-motor therapy in women with Alzheimer's disease: A randomized single-blind pilot study.

Scientific reports·2026
Same journal

Hallmarks of social action in the vocal turn-taking of wild common marmosets (Callithrix jacchus).

Scientific reports·2026
Same journal

Role and mechanism of AOPPs-induced NOX4-mediated ferroptosis in intervertebral disc degeneration.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Published on: October 6, 2019

7.0K

Two-Layer Elastographic 3-D Traction Force Microscopy.

Begoña Álvarez-González1,2, Shun Zhang2, Manuel Gómez-González2

  • 1Division of Cell and Developmental Biology, University of California, San Diego.

Scientific Reports
|January 12, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces two-layer elastographic TFM (2LETFM), a new method to simultaneously measure cell forces and substratum Poisson

More Related Videos

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

6.5K
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

7.5K

Related Experiment Videos

Last Updated: Mar 9, 2026

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Published on: October 6, 2019

7.0K
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

6.5K
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

7.5K

Area of Science:

  • Biophysics
  • Cell Mechanics
  • Materials Science

Background:

  • Cellular traction force microscopy (TFM) quantifies cell-generated forces by measuring substratum deformation.
  • Accurate TFM requires precise knowledge of the substratum's mechanical properties, particularly its Poisson's ratio.
  • Existing TFM methods are sensitive to inaccuracies in Poisson's ratio, potentially affecting stress calculations.

Purpose of the Study:

  • To develop a novel method for simultaneously measuring cell-generated forces and substratum mechanical properties.
  • To introduce two-layer elastographic TFM (2LETFM) to overcome limitations of conventional TFM.
  • To validate the accuracy and robustness of 2LETFM through in silico and experimental analyses.

Main Methods:

  • Developed two-layer elastographic TFM (2LETFM) utilizing an analytical solution of the elastostatic equation.
  • Employed deformation measurements from two distinct substratum layers to determine Poisson's ratio and cell forces.
  • Validated the method using in silico simulations and experimental studies with migrating Physarum amoeboae.

Main Results:

  • 2LETFM accurately and simultaneously measures cell-generated forces and substratum Poisson's ratio.
  • In silico analysis demonstrated 2LETFM's robustness against experimental variations and measurement noise.
  • Experimental proof of principle confirmed the method's efficacy in live cell studies.

Conclusions:

  • 2LETFM offers a significant advancement for TFM by enabling concurrent measurement of mechanical properties and cell forces.
  • This method enhances the reliability of traction force microscopy, particularly in complex cellular environments.
  • 2LETFM opens new avenues for studying cell-matrix interactions and mechanobiology.