A highly selective fluorescent probe for Fe³⁺ in living cells: a stress induced cell based model study

A rhodamine-phenanthroline dyad based fluorescent probe 4 has been designed and synthesized which selectively monitors Fe³⁺ ions among the various metal ions tested. Furthermore, probe 4 has been explored for monitoring of dynamic changes in the Fe³⁺ ion level under aggressive Fenton reaction conditions using a cell based model study. More significantly, probe 4 has also been utilized for real time imaging of endogenous Fe³⁺ ions in living C6 cell lines, the results of which demonstrated that probe 4 acts as an efficient fluorescent tool for Fe³⁺ ion detection in biological systems.