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Assay for Transposase-Accessible Chromatin Using Sequencing (ATAC-seq) Data Analysis.

Kristy L S Miskimen1, E Ricky Chan2, Jonathan L Haines1,2

  • 1Department of Epidemiology and Biostatistics, Case Western Reserve University, Cleveland, Ohio.

Current Protocols in Human Genetics
|January 12, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) method for analyzing epigenetics in human cells. It details sample processing and data analysis for reliable chromatin accessibility insights.

Keywords:
ATAC-seqchromatin accessibilityepigeneticspeak-calling

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Area of Science:

  • Genomics
  • Epigenetics
  • Molecular Biology

Background:

  • Epigenetic modifications significantly influence human gene expression.
  • Understanding chromatin structure is crucial for studying gene regulation.

Purpose of the Study:

  • To present a comprehensive data analysis plan for ATAC-seq.
  • To describe the processing of primary human blood samples for ATAC-seq.
  • To outline quality control measures for ATAC-seq data integrity.

Main Methods:

  • Developed a rapid Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq).
  • Method requires a low input of living cells.
  • Protocol includes processing of primary human blood samples.

Main Results:

  • The ATAC-seq method provides a way to examine chromatin accessibility across the epigenome.
  • Established procedures for sample preparation and data analysis.
  • Identified key quality control parameters for reliable results.

Conclusions:

  • The described ATAC-seq protocol is efficient for analyzing epigenomic landscapes.
  • Ensures data integrity and confidence in epigenetic studies.
  • Facilitates the study of gene expression regulation through chromatin accessibility.