Related Experiment Video
Updated: Mar 9, 2026
05:47
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
32.4K
Infection Programs Sustained Lymphoid Stromal Cell Responses and Shapes Lymph Node Remodeling upon Secondary
Julia L Gregory1, Anne Walter1, Yannick O Alexandre1
1Department of Microbiology and Immunology, The University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
Cell Reports
|January 12, 2017
Summary
Lymphoid stromal cells (LSCs) in lymph nodes (LNs) expand during viral infection, altering LN structure. This persistent change can impact future immune responses to new infections.
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Lymph nodes (LNs) house immune cells and are crucial for adaptive immunity.
- The role of lymphoid stromal cells (LSCs) in regulating lymph node structure and immune response dynamics is not fully understood.
Purpose of the Study:
- To investigate the functional and transcriptional responses of LSCs during skin viral infection.
- To determine how LSC responses impact lymph node remodeling and subsequent immune surveillance.
Main Methods:
- Comprehensive functional and transcriptional analysis of LSC responses in a mouse model of skin viral infection.
- Assessment of cell recruitment, LSC proliferation, and transcriptional changes post-infection.
- Evaluation of lymph node composition and LSC responsiveness to secondary infection.
Main Results:
- LSC subsets exhibited robust and pathogen-specific responses to viral infection.
- Recruitment of immune cells induced LSC expansion, sustained by B cells independently of antigen.
- Infection triggered transient LSC transcriptional changes, but persistent expansion of fibroblastic reticular cell networks occurred.
- Altered lymph node composition due to LSC expansion reduced the magnitude of responses to subsequent infections.
Conclusions:
- LSC responses to infection are complex, involving rapid transcriptional changes and sustained structural remodeling.
- Persistent alterations in lymph node stromal cell networks can modulate immune memory and influence responses to subsequent infections.
Related Concept Videos
01:23Cells of the Adaptive Immune Response
9.7K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
9.7K
01:15Secondary Lymphoid Organs
9.9K
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
9.9K
01:24B Cell Activation and Differentiation
17.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.6K
01:40Cell-mediated Immune Responses
85.4K
Overview
85.4K
01:18Lymphoid Cells and Tissues
3.6K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.6K
01:28Functions of the Lymphatic and Immune System
7.5K
The lymphatic system plays a crucial role in bolstering our immune system. It consists of a network of lymphoid organs, lymph, and lymphatic vessels that provide structural and functional support in safeguarding the body against pathogens such as viruses and bacteria.
The primary lymphoid organs, including the bone marrow and the thymus, serve as the maturation sites for lymphocytes. Secondary lymphoid organs, like the mucosa-associated lymphoid tissue, activate these lymphocytes and serve as...
The primary lymphoid organs, including the bone marrow and the thymus, serve as the maturation sites for lymphocytes. Secondary lymphoid organs, like the mucosa-associated lymphoid tissue, activate these lymphocytes and serve as...
7.5K