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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

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Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease

Nóra Judit Béres1, Zoltán Kiss1, Zsófia Sztupinszki1

  • 11st Department of Pediatrics, Semmelweis University, Budapest, Hungary.

Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
|January 13, 2017
PubMed
Abstract

Insights

MicroRNAs (miRs) show distinct patterns in pediatric inflammatory bowel diseases (IBD). This research identified specific miR profiles in Crohn's disease (CD) and ulcerative colitis (UC) that could aid in diagnosis.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRs) are emerging as key players in the pathogenesis of inflammatory bowel diseases (IBD).
  • Understanding the role of miRs in IBD offers novel research avenues and potential diagnostic insights.

Purpose of the Study:

  • To identify a characteristic microRNA (miR) profile in pediatric IBD (pIBD).
  • To differentiate between Crohn's disease (CD) and ulcerative colitis (UC) using specific miR patterns.
  • To analyze the target genes associated with differentially expressed miRs in pIBD.

Main Methods:

  • Small RNA sequencing on colonic biopsies from pediatric CD patients and controls.
  • RT-PCR validation of selected miRs, including patients with ulcerative colitis (UC).
  • Bioinformatic analysis using MiRTarBase and transcriptome data to identify miR-target gene networks.

Main Results:

  • Sequencing revealed 170 differentially expressed miRs.
  • RT-PCR identified miR-31, -125a, -142-3p, and -146a as discriminators between inflamed CD and UC mucosa.
  • Distinct miR expression patterns were observed in the intact mucosa of CD patients compared to controls, with specific miRs elevated exclusively in CD.

Conclusions:

  • A characteristic colonic miR signature exists in pediatric IBD.
  • This miR pattern may enhance the understanding of IBD pathomechanisms.
  • The identified miR profiles hold potential as a diagnostic tool for pediatric IBD subtypes.
Keywords:
Inflammatory bowel diseaseMicroRNAMucosalPediatric

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