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Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages.

Giovanni Fusco¹, Paolo Bollella², Franco Mazzei²

¹Department of Chemistry and Drug Technologies, Sapienza University of Rome, Rome, Italy; Department of Chemistry, Sapienza University of Rome, Rome, Italy.

Journal of Analytical Methods in Chemistry

|January 13, 2017

Summary

A new biosensor using catalase and nanomaterials detects hydrogen peroxide (H2O2) in beverages. This enhanced electrode offers improved performance and stability for accurate H2O2 measurements.

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Area of Science:

Electrochemistry
Nanomaterials Science
Biosensor Technology

Background:

Hydrogen peroxide (H2O2) is a key analyte in various industrial and biomedical applications.
Developing sensitive and stable biosensors for H2O2 detection is crucial.
Nanocomposite materials offer unique properties for biosensor enhancement.

Purpose of the Study:

To develop a novel catalase-based nanocomposite film modified electrode for H2O2 detection.
To characterize the electrochemical properties of the developed biosensor.
To evaluate the biosensor's performance for H2O2 determination in packaged beverages.

Main Methods:

Fabrication of a glassy carbon electrode modified with a catalase-based (NAF/MWCNTs) nanocomposite film.
Electrochemical characterization using cyclic voltammetry (CV).
Application of modification steps to mesoporous graphite screen-printed electrodes.

Main Results:

The nanocomposite film enhanced the bioelectrochemical properties of the electrode.
Electron transfer coefficient (α) and rate constant (k) were determined.
The modified biosensor exhibited a linear response in the range 2.5–1150 μmol L⁻¹ with a limit of detection (LOD) of 0.83 μmol L⁻¹.
Nafion and carbon nanotubes maintained the biological activity of catalase.

Conclusions:

The developed NAF/MWCNTs modified electrode is a promising tool for sensitive H2O2 detection.
The biosensor demonstrates excellent performance and stability.
Potential application for H2O2 determination in packaged beverages.