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Fluorescent Carbon Dot as Nanosensor for Sensitive and Selective Detection of Cefixime Based on Inner Filter Effect
Farhad Akhgari1, Naser Samadi2, Khalil Farhadi1
1Department of Analytical Chemistry, Faculty of Chemistry, Urmia University, Urmia, Iran.
Journal of Fluorescence
|January 13, 2017
Summary
A new fluorescent assay detects Cefixime using carbon dots and palladium ions via an inner filter effect. This sensitive method accurately quantifies Cefixime in milk and urine samples.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Cefixime (CEF) is a widely used antibiotic.
- Existing fluorescent assays for Cefixime have limitations.
- Novel sensing platforms are needed for sensitive and selective detection.
Purpose of the Study:
- To develop a simple, sensitive, and novel fluorescent assay for Cefixime detection.
- To utilize the inner filter effect (IFE) for Cefixime quantification.
- To apply the developed method for Cefixime determination in real-world samples.
Main Methods:
- Synthesized fluorescent carbon dots (CDs) via one-pot method.
- Employed an inner filter effect (IFE) sensing strategy.
- Utilized the complexation of Cefixime with Palladium (II) ions.
Main Results:
- The Pd(II)-CEF complex acted as an effective absorber, quenching CDs fluorescence.
- A good linear relationship was observed between 0.2 × 10⁻⁶ M and 8 × 10⁻⁶ M (R² = 0.987).
- A low detection limit of 0.5 × 10⁻⁷ M was achieved.
Conclusions:
- The developed IFE-based assay is a simple and sensitive method for Cefixime detection.
- The assay demonstrated successful application in raw milk and human urine samples.
- This approach offers a promising alternative for Cefixime analysis.
