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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
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High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

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Insights into nuclear dynamics using live-cell imaging approaches.

Rachel B Bigley1, Alexander Y Payumo1, Jeffrey M Alexander1

  • 1Cardiovascular Research Institute and Department of Physiology, School of Medicine, University of California, San Francisco, CA, USA.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine
|January 13, 2017
PubMed
Summary
This summary is machine-generated.

Live-cell imaging offers a dynamic view of nuclear processes, overcoming the limitations of static

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Single-Molecule Imaging of Nuclear Transport
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Area of Science:

  • Cell Biology
  • Systems Biology
  • Genetics

Background:

  • The cell nucleus houses the genetic blueprint and regulates gene expression through complex interactions.
  • Current methods like omics provide static snapshots, limiting understanding of dynamic nuclear processes.
  • Studying molecules in their native, living contexts is crucial for a holistic view of nuclear dynamics.

Purpose of the Study:

  • To review how live-cell imaging advances the study of nuclear dynamics.
  • To highlight the capabilities of live-cell imaging in assessing gene regulatory interactions quantitatively.
  • To explore recent insights from live-cell imaging in understanding nuclear organization and function.

Main Methods:

  • Live-cell imaging techniques are employed to observe molecular dynamics in real-time.
  • Quantitative assessments of molecular interactions within the nucleus are performed.
  • Focus on interactions involving DNA, proteins, RNA, and the nuclear envelope.

Main Results:

  • Live-cell imaging provides dynamic, quantitative data on nuclear processes.
  • This approach overcomes the limitations of static 'omic' snapshots.
  • Enables visualization of interactions critical for gene regulation in living cells.

Conclusions:

  • Live-cell imaging is essential for a comprehensive understanding of nuclear dynamics.
  • It allows for the study of molecular interactions in their native cellular environment.
  • Advances in live-cell imaging are transforming the study of gene regulation and nuclear organization.