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Updated: Mar 8, 2026
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Evaluation of Herpes Simplex Virus Type 2 Serological Tests for Use With Dried Blood Spots in Kenya
Marcia M Hobbs1, Sophie W Mwanyumba, Winnie K Luseno
1From the *Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC; †National HIV Reference Laboratory, Ministry of Health, Nairobi, Kenya; ‡Pacific Institute for Research and Evaluation; and §Department of Maternal and Child Health, University of North Carolina at Chapel Hill, Chapel Hill, NC.
Abstract:
We evaluated 2 assays to detect antibodies to herpes simplex virus type 2 in dried blood spots prepared from blood specimens submitted to a reference laboratory in Kenya. Dried blood spots did not perform well with the Kalon herpes simplex virus type 2 assay. Focus HerpeSelect 2 was 98.8% sensitive and 98.9% specific with dried blood spots.
