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Updated: Mar 8, 2026

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Published on: November 16, 2018

Direct Gingival Fibroblast/Osteoblast Transdifferentiation via Epigenetics.

Y Cho^1,2, B Kim¹, H Bae¹

¹1 Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, South Korea.

Journal of Dental Research

|January 13, 2017

Summary

This summary is machine-generated.

Epigenetic modification can reprogram human gingival fibroblasts into bone-forming osteoblasts. This novel approach shows promise for regenerating alveolar bone lost due to periodontal disease or trauma.

Keywords:

bone regeneration cell differentiation gingiva periodontal diseases periodontium tissue engineering

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors

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Area of Science:

Regenerative Medicine
Epigenetics
Dental Research

Background:

Alveolar bone resorption presents a significant clinical challenge.
Current treatments for bone loss have limitations.

Purpose of the Study:

To investigate the potential of epigenetic modification to transdifferentiate human gingival fibroblasts (HGFs) into functional osteoblasts.
To evaluate the efficacy of this approach for alveolar bone regeneration in vitro and in vivo.

Main Methods:

HGFs were treated with 5-aza-2'-deoxycytidine (5-aza-dC) to induce demethylation of osteogenic genes.
Bone morphogenetic protein 2 (BMP2) was used to promote osteogenic differentiation.
Osteoblastic differentiation was confirmed using cell morphology, ALP and ARS staining, RT-PCR, MSP, and ChIP assays.
In vivo efficacy was assessed via micro-CT and H&E staining after transplantation in mice.

Main Results:

Epigenetic modification with 5-aza-dC and BMP2 treatment successfully induced osteogenic transdifferentiation of HGFs.
Demethylation of RUNX2 and ALP gene promoters was observed, leading to altered gene expression.
In vivo studies demonstrated significant bone formation and high-density signals in epigenetically modified HGF grafts.

Conclusions:

Epigenetic reprogramming offers a direct pathway to convert HGFs into functional osteoblasts.
This method holds potential as a novel therapeutic strategy for alveolar bone regeneration.