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Highly sensitive quantification for human plasma-targeted metabolomics using an amine derivatization reagent.

Naoko Arashida1, Rumi Nishimoto1, Masashi Harada1

  • 1Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.

Analytica Chimica Acta
|January 14, 2017
PubMed
Summary

This study presents a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for accurately quantifying 40 physiological amines in plasma. The developed method enables sensitive and rapid analysis of these important biomarkers for disease detection.

Keywords:
DerivatizationHigh performance liquid chromatographyPlasmaTandem mass spectrometryTargeted metabolomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Chemistry

Background:

  • Physiological amines are crucial biomarkers for various diseases.
  • Existing quantification methods are limited to major compounds, hindering comprehensive analysis.
  • Previous work established a sensitive LC-MS/MS method for amine quantification.

Purpose of the Study:

  • To develop a practical and accurate LC-MS/MS method for analyzing low concentrations of 40 physiological amines.
  • To achieve simultaneous quantification within a 19-minute timeframe.
  • To improve the detection and measurement of amine metabolites in biological samples.

Main Methods:

  • Utilized 3-aminopyridyl-N-succinimidyl carbamate derivatization.
  • Employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) with scheduled selected reaction monitoring.
  • Validated the method for linearity, limit of quantification, accuracy, precision, and recovery in plasma.

Main Results:

  • Successfully developed a method to analyze 40 physiological amines in 19 minutes.
  • Demonstrated good analytical characteristics for 35 amines in plasma samples.
  • Quantified 24 out of 27 detected amines in plasma from healthy volunteers with high accuracy across three orders of magnitude.

Conclusions:

  • The new LC-MS/MS method provides a practical and accurate approach for quantifying a wide range of physiological amines.
  • This method enhances the ability to use amine metabolites as biomarkers for disease diagnosis and monitoring.
  • The validated method supports high-throughput analysis of clinically relevant amine concentrations in biological matrices.