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Curculactones A and B induced the differentiation of C3H10T1/2 and MC3T3-E1 cells to osteoblasts
Hyo-Eun Son1, Tae Hoon Kim2, Won-Gu Jang1
1Department of Biotechnology, School of Engineering, Daegu University, Gyeongsan 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongsan 38453, Republic of Korea.
Abstract:
Curculactones A and B are rare γ-lactone derivatives obtained from yellow, natural curcumin following γ-irradiation, and are a type of small molecules with a moderate anti-obesity effect. However, the exact role of curculactones A and B in osteoblast differentiation is unknown. In this study, the effects of curculactones A and B on the differentiation of the mesenchymal cell line C3H10T1/2 and pre-osteoblast cell line MC3T3-E1 to osteoblasts were examined. Curculactones A or B could markedly increase the mRNA levels of osteogenic marker genes and alkaline phosphatase (ALP) activity. Collectively, our findings indicate that curculactones A or B induced osteoblast differentiation through osteogenic expression of genes such as distal-less homeobox 5 (Dlx5), runt-related transcription factor 2 (Runx2), ALP, and osteocalcin (OC).
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