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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Published on: January 17, 2014
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The application of phenotypic microarray analysis to anti-fungal drug development
Darren Greetham1, David F Lappin2, Ranjith Rajendran2
1School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
Journal of Microbiological Methods
|January 14, 2017
Abstract:
Candida albicans metabolic activity in the presence and absence of acetylcholine was measured using phenotypic microarray analysis. Acetylcholine inhibited C. albicans biofilm formation by slowing metabolism independent of biofilm forming capabilities. Phenotypic microarray analysis can therefore be used for screening compound libraries for novel anti-fungal drugs and measuring antifungal resistance.

