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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Transposons01:24

Transposons

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Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
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Related Experiment Video

Updated: Mar 8, 2026

piggyBac Transposon System Modification of Primary Human T Cells
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piggyBac Transposon System Modification of Primary Human T Cells

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An optimized, broadly applicable piggyBac transposon induction system.

Zongtai Qi1, Michael Nathaniel Wilkinson1, Xuhua Chen1

  • 1Department of Genetics and Center for Genome Sciences and Systems Biology, Washington University, School of Medicine, St. Louis, MO 63108, USA.

Nucleic Acids Research
|January 14, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a controllable piggyBac (PB) transposon system using FKBP-DD technology. This FKBP-DD system offers improved control over transposition, enabling precise genome engineering applications.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The piggyBac (PB) transposon is a valuable tool in molecular biology.
  • Controlling PB transposon insertion is crucial for many experimental applications.
  • Existing methods lack precise temporal and spatial control.

Purpose of the Study:

  • To systematically characterize post-translational control methods for the PB transposon.
  • To evaluate the efficiency and dynamic range of different control systems.
  • To assess the impact of chemical inducers on cellular function.

Main Methods:

  • Fusing PB transposase with ERT2, FKBP-DD, and DHFR-DD domains.
  • Testing constructs in four different cell lines.
  • Measuring fold-induction, transposition efficiency, and cellular effects of inducers.

Main Results:

  • FKBP-DD system demonstrated higher transposition activity and dynamic range compared to others.
  • FKBP-DD mediated reversible and dose-dependent control of PB transposon activity.
  • Shld1 (FKBP-DD inducer) did not affect stem cell differentiation, unlike tamoxifen.

Conclusions:

  • The FKBP-DD controlled PB transposon system provides robust and reversible regulation.
  • This system is suitable for applications like genome engineering and insertional mutagenesis.
  • FKBP-DD system offers advantages over existing PB transposon control methods.