Distribution of Hydrogenases in Cyanobacteria: A Phylum-Wide Genomic Survey

Microbial Molecular hydrogen (H₂) cycling plays an important role in several ecological niches. Hydrogenases (H₂ases), enzymes involved in H₂ metabolism, are of great interest for investigating microbial communities, and producing BioH₂. To obtain an overall picture of the genetic ability of Cyanobacteria to produce H₂ases, we conducted a phylum wide analysis of the distribution of the genes encoding these enzymes in 130 cyanobacterial genomes. The concomitant presence of the H₂ase and genes involved in the maturation process, and that of well-conserved catalytic sites in the enzymes were the three minimal criteria used to classify a strain as being able to produce a functional H₂ase. The [NiFe] H₂ases were found to be the only enzymes present in this phylum. Fifty-five strains were found to be potentially able produce the bidirectional Hox enzyme and 33 to produce the uptake (Hup) enzyme. H₂ metabolism in Cyanobacteria has a broad ecological distribution, since only the genomes of strains collected from the open ocean do not possess hox genes. In addition, the presence of H₂ase was found to increase in the late branching clades of the phylogenetic tree of the species. Surprisingly, five cyanobacterial genomes were found to possess homologs of oxygen tolerant H₂ases belonging to groups 1, 3b, and 3d. Overall, these data show that H₂ases are widely distributed, and are therefore probably of great functional importance in Cyanobacteria. The present finding that homologs to oxygen-tolerant H₂ases are present in this phylum opens new perspectives for applying the process of photosynthesis in the field of H₂ production.