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Genome-wide DNA methylation profile in mungbean
Yang Jae Kang1, Ahra Bae1, Sangrea Shim1
1Department of Plant Science and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
Scientific Reports
|January 14, 2017
Summary
This study reveals whole-genome DNA methylation profiles in two mungbean cultivars, linking DNA methylation patterns and single nucleotide polymorphisms (SNPs) to gene expression and phenotypic variations for molecular breeding.
Area of Science:
- Plant genomics
- Epigenetics
- Crop science
Background:
- DNA methylation influences gene expression and phenotypic variation in crops.
- Understanding DNA methylation is crucial for crop improvement and molecular breeding.
Purpose of the Study:
- To present whole-genome DNA methylation profiles for two mungbean cultivars: Sunhwanogdu (VC1973A) and Kyunggijaerae#5 (V2984).
- To assess the impact of single nucleotide polymorphisms (SNPs) on DNA methylation.
- To correlate DNA methylation patterns with gene expression.
Main Methods:
- Whole-genome DNA methylation profiling using bisulfite sequencing (BSseq) on leaf tissues.
- Gene expression analysis using RNA sequencing (RNAseq).
- Analysis of single nucleotide polymorphisms (SNPs) and their effect on methylation.
Main Results:
- DNA methylation patterns and frequencies were characterized in both mungbean cultivars.
- Specific single nucleotide polymorphisms (SNPs) affecting CHH methylation were identified.
- DNA methylation was found to control transcript expression of paralogous genes and correlate with differential gene expression between cultivars.
Conclusions:
- Mungbean genome-wide methylation profiles provide insights into phenotypic variations.
- Findings support the role of DNA methylation in regulating gene expression and cultivar differences.
- This resource is valuable for molecular breeding strategies in mungbeans.
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