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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
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A cell-permeable and triazole-forming fluorescent probe for glycoconjugate imaging in live cells
Jiun-Jie Shie1, Ying-Chih Liu2, Jye-Chian Hsiao1
1Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. shiejj@gate.sinica.edu.tw.
Abstract:
A new fluorescence-forming probe, coumOCT, designed by fusing cyclooctyne with a coumarin fluorophore was successfully used for the imaging of azido-glycoconjugates in living HeLa cells. This probe is cell-permeable and generates fluorescence after triazole formation, thus minimizing the background signal and enabling the real-time intracellular imaging of glycoconjugate trafficking.

