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Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing

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Area of Science:

Optoelectronics
Biotechnology
Nanotechnology

Background:

Optical fiber sensors offer potential for sensitive detection.
Aptamers provide specific molecular recognition capabilities.
Plasmonic devices enhance optical sensing performance.

Purpose of the Study:

To develop a generic opto-bio-sensing platform combining aptamers and plasmonics.
To achieve high index spectral sensitivity and attomolar detection limits.
To demonstrate real-time kinetic studies in aqueous solutions.

Main Methods:

Immobilization of aptamers on a nano-structured thin film plasmonic sensing device.
Utilizing an optical fiber platform for signal transduction.
Detection of thrombin in solution and flow cell experiments.

Main Results:

Achieved high index spectral sensitivity (3.4 × 10^4 nm/RIU) with a figure of merit of 330.
Demonstrated attomolar (50 aM) detection of thrombin in 1 mm^3 solution.
Successfully detected nanomolar thrombin in the presence of albumin, covering the human thrombin generation curve.

Conclusions:

The developed platform offers a single-stage, solution-phase, attomolar detection capability.
The sensor enables real-time kinetic studies in water-based chemistry.
The platform is suitable for multiplexed and remote sensing applications.