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An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores.

Shaoliang Dai1, Shijia Wu2, Nuo Duan3

  • 1State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Taicang Entry-Exit Inspection and Quarantine Bureau, Suzhou 215400, China.

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We created a sensitive aptasensor for detecting Ochratoxin A (OTA) in beverages. This novel method uses nanoparticles and graphene oxide for highly accurate Ochratoxin A (OTA) quantification.

Keywords:
Graphene oxideLuminescence resonance energy transferOchratoxin AUpconversion nanoparticles

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Ochratoxin A (OTA) is a harmful mycotoxin found in food and beverages.
  • Accurate detection of OTA is crucial for food safety and public health.
  • Existing detection methods may lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a highly sensitive and specific aptasensor for Ochratoxin A (OTA) detection.
  • To utilize core/shell upconversion nanoparticles (CS-UCNPs) and graphene oxide (GO) for enhanced sensing.
  • To validate the aptasensor's performance in complex matrices like beer.

Main Methods:

  • Fabrication of CS-UCNPs as luminophores and energy donors.
  • Conjugation of OTA aptamer to CS-UCNPs.
  • Utilizing graphene oxide (GO) as an energy acceptor.
  • Employing luminescence resonance energy transfer (LRET) for signal generation.
  • Establishing a calibration curve for OTA quantification.

Main Results:

  • The aptasensor demonstrated ultrahigh sensitivity with a detection limit of 0.001 ng/mL.
  • A wide linear range (0.001–250 ng/mL) was achieved for OTA detection.
  • The aptasensor exhibited excellent specificity for OTA in beer samples.
  • The assay showed robustness in the complex beer sample matrix.

Conclusions:

  • The developed LRET aptasensor offers a promising tool for sensitive and reliable OTA detection.
  • Its high sensitivity and specificity make it suitable for practical food safety applications.
  • This nanotechnology-based approach provides a valuable alternative for mycotoxin analysis.