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Assessment of GAPDH expression by quantitative real time PCR in blood of Moroccan AD cases
Nadia El Kadmiri1, Meryam El Khachibi2, Ilham Slassi3
1IBN ZOHR University, Faculté Polydisciplinaire de Taroudant, B.P: 271, 83 000 Taroudant, Morocco; Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco.
Introduction:
Neuroproteomics studies have showed the high affinity interactions between GAPDH - β-amyloid in Alzheimer disease. The aim of our study is to complete our previous studies by assessing the mechanism responsible of decreased expression of GAPDH protein in the blood of Moroccan AD cases probably due to an alteration at the transcriptional level or at the post translational level.
Methods:
The mRNA expression of GAPDH was assessed by quantitative real time PCR in AD cases and healthy controls.
Results:
Our result revealed a significant difference of mRNA expression level of GAPDH in AD cases as compared to healthy controls (P<0.05).
Conclusion:
This data is consistent with several studies by showing the direct involvement of GAPDH in amyloid aggregation by undergoing several modifications, which influence its chemical structure and its biological activity.
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