Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

1.8K
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
1.8K
Lipid Absorption01:24

Lipid Absorption

3.2K
Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
3.2K
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

6.3K
Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
6.3K
Lipid Digestion01:06

Lipid Digestion

101.3K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
101.3K
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

7.2K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
7.2K
Lipid Catabolism01:25

Lipid Catabolism

1.3K
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aberrant CD4<sup>+</sup> T cell refeeding response impairs neuro-immune crosstalk in Parkinson's disease.

bioRxiv : the preprint server for biology·2026
Same author

Amplification of genetic and metabolic factors in alpha-1 antitrypsin deficiency.

Hepatology (Baltimore, Md.)·2026
Same author

Patient Experience and Perception of First Language Usage in Healthcare: The Welsh Perspective.

Journal of patient experience·2026
Same author

Current mass spectrometry methods for the analysis of 7-ketocholesterol and related sterols.

The Journal of steroid biochemistry and molecular biology·2026
Same author

Seizure classification using a multimodal seizure monitoring system (Nelli) in Dravet and Lennox-Gastaut syndromes: A non-randomized, single-center feasibility study.

Epilepsia·2025
Same author

Preventing Alcohol-Related Harm: Effective Strategies and the Role of Health Professionals.

British journal of hospital medicine (London, England : 2005)·2025
Same journal

The Long Run: A Tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
Same journal

A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines.

Analytical biochemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 8, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.8K

Cholesterolomics: An update.

William J Griffiths1, Jonas Abdel-Khalik1, Eylan Yutuc1

  • 1Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK.

Analytical Biochemistry
|January 15, 2017
PubMed
Summary
This summary is machine-generated.

Cholesterolomics studies cholesterol and its metabolites, focusing on bile acid pathway intermediates like oxysterols. These compounds are linked to various diseases, and mass spectrometry is key for their analysis.

Keywords:
Cholestenoic acidsCholesterolDerivatisationMass spectrometryOxysterols

More Related Videos

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

6.7K
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.7K

Related Experiment Videos

Last Updated: Mar 8, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.8K
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

6.7K
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.7K

Area of Science:

  • Biochemistry
  • Metabolomics
  • Analytical Chemistry

Background:

  • Cholesterolomics involves identifying and quantifying cholesterol, its precursors, and metabolites in biological samples.
  • Key molecules include vitamin D, steroid hormones, bile acids, and their biosynthetic intermediates.
  • Oxysterols and cholestenoic acids are critical intermediates in bile acid biosynthesis.

Purpose of the Study:

  • To focus on oxysterols and cholestenoic acids within bile acid biosynthesis pathways.
  • To highlight the link between these sterol intermediates and various diseases.
  • To discuss recent analytical improvements in sterol analysis.

Main Methods:

  • Mass spectrometry (MS) is the primary technology for sterol analysis.
  • Techniques include gas-chromatography-MS (GC-MS) and liquid chromatography-MS (LC-MS).
  • Matrix-assisted laser desorption/ionisation (MALDI)-MS is used for tissue imaging.

Main Results:

  • Oxysterols and cholestenoic acids are implicated in diseases such as autoimmune disorders, Parkinson's disease, and breast cancer.
  • These sterol intermediates play a role in Niemann-Pick type C and Smith-Lemli-Opitz syndrome.
  • Recent analytical advancements enhance the study of these molecules.

Conclusions:

  • Cholesterolomics, particularly the analysis of bile acid intermediates, offers insights into disease aetiology.
  • Mass spectrometry techniques are crucial for the accurate identification and quantification of these sterols.
  • Further research into oxysterols and cholestenoic acids holds promise for understanding and potentially treating related diseases.