Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.0K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.0K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

10.0K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
10.0K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.7K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bile acids as a predictor for all-cause mortality in peritoneal dialysis patients: a retrospective cohort study.

BMC nephrology·2026
Same author

GliomaDeconv Maps Glioma-Specific Cellular Programs from Bulk Transcriptomic Profiles and Reveals Spatiotemporal Associations with Progression and Treatment Response.

Cancer letters·2026
Same author

Age-related odor and design buffering in elderly solo housing: two-stage study of visitor behavioral intention.

Scientific reports·2026
Same author

iRGD-modified peptide-drug conjugate improves brain delivery and antitumor efficacy in glioblastoma.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Positive-Strand RNA Viral RdRps: From Structure Conservation and Activity Assay to Drug Development.

Molecules (Basel, Switzerland)·2026
Same author

Biomimetic Macrophage Cell Membrane-Based Nanoparticles for Effective Treatment of Glioblastoma Through Boron Neutron Capture Therapy Combined With Immunotherapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Mar 8, 2026

Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

23.9K

Identification and characterization of cell-bound membrane vesicles.

Qisheng Tang1, Xiaojun Zhang2, Wendiao Zhang2

  • 1Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi, PR China; College of Life Sciences, Nanchang University, Nanchang, Jiangxi, PR China.

Biochimica Et Biophysica Acta. Biomembranes
|January 16, 2017
PubMed
Summary

Cell-bound membrane vesicles on endothelial and hepatoma cells are novel structures, distinct from extracellular vesicles and intracellular organelles. These unique vesicles possess lipid raft components and detergent resistance, suggesting unknown molecular compositions.

Keywords:
Detergent resistanceHuman hepatoma HepG-2 cellsHuman umbilical vein endothelial cells (HUVECs)Lipid raftsMembrane vesicles

More Related Videos

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.9K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

5.8K

Related Experiment Videos

Last Updated: Mar 8, 2026

Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

23.9K
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.9K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

5.8K

Area of Science:

  • Cell Biology
  • Membrane Biology
  • Biochemistry

Background:

  • Extracellular vesicles are well-characterized, but cell-bound membrane vesicles remain poorly understood.
  • Existing markers for extracellular vesicles and intracellular organelles do not identify cell-bound vesicles.
  • Investigating these cell-bound structures is crucial for understanding cellular processes.

Purpose of the Study:

  • To identify and characterize cell-bound membrane vesicles on human umbilical vein endothelial cells (HUVECs) and human hepatoma HepG-2 cells.
  • To differentiate cell-bound vesicles from extracellular vesicles and intracellular organelles.
  • To elucidate the biochemical properties and potential composition of cell-bound membrane vesicles.

Main Methods:

  • Microscopy and co-localization studies using specific markers for extracellular vesicles, intracellular organelles, and lipid rafts.
  • Differential detergent extraction assays to assess resistance to various detergents (Triton X-100, SDS, saponin).
  • Solubility tests using organic solvents (chloroform-methanol, ethanol) and lipid-stabilizing fixatives (OsO4).

Main Results:

  • Cell-bound membrane vesicles were not co-localized with markers for extracellular vesicles (e.g., phosphatidylserine, CD63) or intracellular organelles (e.g., mitochondria, lysosomes).
  • These vesicles exhibited resistance to multiple detergents but were soluble in organic solvents, suggesting a lipid component.
  • Cell-bound vesicles were co-localized with lipid raft markers (caveolin-1, GM1), indicating their presence within these membrane domains.

Conclusions:

  • Cell-bound membrane vesicles represent a novel plasma membrane structure, distinct from extracellular vesicles and intracellular organelles.
  • Their detergent resistance and lipid raft association suggest unique lipid compositions and potentially unknown protein components.
  • Further research requires effective isolation and purification methods to fully characterize these unique cellular structures.