Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Species-Specific Isotope Dilution Methodology for the SI Traceable Quantification of Serum Human Ferritin Light Chain (FTL).

Analytical chemistry·2025
Same author

Lithographically Controlled Liquid Metal Diffusion in Graphene: Fabrication and Magnetotransport Signatures of Superconductivity.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Unprecedented accuracy in molecular line-intensity ratios from frequency-based measurements.

Science advances·2025
Same author

Comparative evaluation of peptide vs. protein-based calibration for quantification of cardiac troponin I using ID-LC-MS/MS.

Clinical chemistry and laboratory medicine·2025
Same author

Enquiries into virulent identifications.

The International journal of psycho-analysis·2024
Same author

Controlling Nanoparticle Distance by On-Surface DNA-Origami Folding.

Small (Weinheim an der Bergstrasse, Germany)·2024

Related Experiment Video

Updated: Feb 19, 2026

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
07:04

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology

Published on: January 24, 2025

1.2K

Comparison of potential higher order reference methods for total haemoglobin quantification-an interlaboratory study.

Claudia Frank1, Christine Brauckmann2, Marie Palos3

  • 1Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany. Claudia.Frank@ptb.de.

Analytical and Bioanalytical Chemistry
|January 17, 2017
PubMed
Summary

This study evaluates new methods for measuring total hemoglobin (Hb) concentration, addressing limitations of the conventional cyanmethemoglobin (HiCN) method, including its lack of SI traceability and cyanide toxicity. New approaches show promise for accurate and safer Hb determination.

Keywords:
HaemoglobinHiCNIsotope dilutionMass spectrometryRaman spectroscopyReference method

More Related Videos

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
05:08

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

5.6K
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

10.2K

Related Experiment Videos

Last Updated: Feb 19, 2026

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
07:04

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology

Published on: January 24, 2025

1.2K
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
05:08

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

5.6K
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

10.2K

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Metrology

Background:

  • Total hemoglobin (Hb) concentration is a critical clinical biomarker for assessing human health.
  • The standard spectrophotometric cyanmethemoglobin (HiCN) method lacks SI traceability and uses toxic cyanide.
  • Existing methods do not meet the highest metrological demands for Hb determination.

Purpose of the Study:

  • To investigate and validate alternative methods for total Hb concentration measurement.
  • To address the metrological limitations and toxicity issues associated with the conventional HiCN method.
  • To compare novel analytical techniques against the established HiCN reference method.

Main Methods:

  • Validation of alternative Hb measurement techniques against the HiCN method.
  • Interlaboratory comparison using certified reference material JCCRM 912-2M.
  • Employed methods include spectrophotometric alkaline haematin detergent (AHD) and isotope dilution (ID) techniques such as ICP-MS, SS-ICP-MS, organic MS, and Raman spectrometry.

Main Results:

  • The study validated several alternative methods for total Hb concentration determination.
  • These methods were assessed for their accuracy and reliability compared to the HiCN method.
  • Results indicate potential for improved Hb measurement with enhanced metrological traceability and reduced toxicity.

Conclusions:

  • Alternative methods, including AHD and various ID-based techniques, offer viable solutions to the limitations of the HiCN method.
  • These novel approaches provide pathways for more accurate, traceable, and safer total Hb concentration measurements in clinical diagnostics.
  • Further development and implementation of these methods can enhance the metrological quality of Hb analysis.