Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide
Artem V Maerle1, Daria V Voronina2, Kira L Dobrochaeva2
1JSC NPF DNA-Technology, Moscow, Russia. maerle@yandex.ru.
Abstract:
The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.
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