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A novel polypeptide secreted by activated human T lymphocytes
M D Miller1, S Hata, R De Waal Malefyt
1Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115.
Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1989
Summary
Researchers discovered two novel genes, I-309 and G-26, highly expressed in activated human peripheral blood mononuclear cells (PBMC). These genes encode small, secreted proteins, with I-309 representing a potentially new cytokine.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Activated human peripheral blood mononuclear cells (PBMC) express specific genes.
- Small secreted polypeptides play roles in cellular communication and immune responses.
Purpose of the Study:
- To identify and characterize novel genes expressed in activated human PBMC.
- To investigate the function and nature of the I-309 gene product.
Main Methods:
- cDNA cloning and nucleotide sequence analysis.
- Generation of anti-peptide antiserum for protein detection.
- Analysis of protein expression in activated T cells and transfected COS cells.
Main Results:
- Two cDNA clones, I-309 and G-26, were identified, with high expression in activated PBMC.
- Sequence analysis predicted small, secreted polypeptides with conserved cysteine motifs.
- I-309 represents a novel gene encoding a secreted protein recognized by specific antiserum.
- The I-309 protein shares structural similarities with known cytokines.
Conclusions:
- I-309 and G-26 encode secreted proteins induced by cell activation.
- I-309 is a novel human gene likely encoding a cytokine with an undetermined function.
- These findings contribute to understanding immune cell activation and signaling pathways.