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Related Experiment Video

Updated: Mar 8, 2026

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

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Published on: September 11, 2016

Complex molecular changes induced by chronic progestogens exposure in roach, Rutilus rutilus.

Gabor Maasz¹, Zita Zrinyi¹, Peter Takacs²

¹MTA-ÖK BLI NAP_B Adaptive Neuroethology, MTA-Centre for Ecological Research, Balaton Limnological Institute, 8237 Tihany, Hungary.

Ecotoxicology and Environmental Safety

|January 17, 2017

Summary

Environmental progestogen contamination in fish was studied. Progestogen exposure altered fish physiology and significantly increased the DJ-1 protein in the brain and liver, suggesting DJ-1 as a sensitive biomarker for aquatic pollution.

Keywords:

DJ-1 protein Fish Progestogens Roach Steroid hormones Vitellogenin

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Published on: September 15, 2012

Area of Science:

Environmental Science
Ecotoxicology
Biochemistry

Background:

Progestogens are potent steroids found in natural waters, posing risks to aquatic ecosystems.
Previous studies detected progestogens in Lake Balaton, necessitating research into their effects on aquatic life.

Purpose of the Study:

To investigate the morphological and molecular effects of a progestogen mixture (progesterone, drospirenone, levonorgestrel) on common roach (Rutilus rutilus).
To explore the relationship between progestogen exposure and the DJ-1 chaperon protein.
To identify potential biomarkers for progestogen contamination in freshwater ecosystems.

Main Methods:

Adult roaches were exposed to environmentally relevant and higher concentrations of progestogens (10, 50, 500 ng/L) for 42 days.
Somatic indices, vitellogenin (VTG) levels, and DJ-1 protein concentrations were measured.
Serum biochemistry (cholesterol, LDL, HDL, triglycerides) and brain cell stress markers were analyzed.

Main Results:

Significant alterations in liver and kidney somatic indices were observed across all exposure groups.
The gonadosomatic index decreased significantly in the highest exposure group (500 ng/L).
Vitellogenin (VTG) levels increased significantly at 500 ng/L, while DJ-1 protein levels significantly increased in both brain and liver across all exposure groups.
Serum LDL and cholesterol levels decreased, potentially mediated by DJ-1 stimulated LDL-receptor expression.

Conclusions:

Combined exposure to environmental progestogens induces significant morphological and molecular changes in fish.
The DJ-1 chaperon protein shows potential as a sensitive biomarker for detecting progestogen contamination in freshwater environments.
DJ-1 may play a role in mediating some of the observed biochemical alterations, such as changes in lipid metabolism.