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Related Experiment Video

Updated: Mar 8, 2026

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
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Blastocyst Development in a Single Medium Compared to Sequential Media: A Prospective Study With Sibling Oocytes.

Ioannis A Sfontouris1,2, Efstratios M Kolibianakis3, George T Lainas1

  • 11 Eugonia Assisted Reproduction Unit, Athens, Greece.

Reproductive Sciences (Thousand Oaks, Calif.)
|January 18, 2017
PubMed
Summary

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Global single-step medium significantly improved blastocyst formation rates compared to sequential media in assisted reproduction. This single medium offers better support for developing embryos, enhancing IVF outcomes.

Area of Science:

  • Reproductive Medicine
  • Embryology
  • In Vitro Fertilization (IVF)

Background:

  • Optimizing embryo culture media is crucial for improving IVF success rates.
  • Sequential media systems require timely interventions, potentially introducing variability.
  • Single-step media offer a simplified approach to embryo culture.

Purpose of the Study:

  • To compare blastocyst formation rates between a single-step medium (Global) and sequential media (ISM1/BlastAssist).
  • To evaluate the impact of different culture media on key IVF outcome parameters.

Main Methods:

  • Prospective trial involving 542 metaphase II oocytes from 31 women.
  • Randomized allocation of sibling oocytes to either Global or ISM1/BlastAssist culture.
  • Interrupted culture with media changes on day 3, with embryo transfer on day 5.
Keywords:
GlobalOrigioblastocyst formationembryo culturesequential mediasingle media

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Related Experiment Videos

Last Updated: Mar 8, 2026

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Main Results:

  • Significantly higher blastocyst formation rates on day 5 with Global medium (61.7%) versus ISM1/BlastAssist (37.0%).
  • Similar fertilization and cleavage rates between the two media groups.
  • Higher percentages of good quality blastocysts, blastocysts selected for transfer, and utilization rates observed with Global medium.

Conclusions:

  • Culture in Global single-step medium is associated with superior blastocyst development compared to ISM1/BlastAssist sequential media.
  • The Global medium may provide enhanced support for early embryonic development in IVF.
  • Single-step media represent a promising alternative for optimizing blastocyst development in clinical IVF settings.