[Scanning electron microscopic observation of mouse ascitic carcinoma cells after intraperitoneal chemotherapy with...

The peritoneal surface of the lesion was observed after treatment by scanning electron microscopy (SEM) to evaluate the anticancer effect of Cisplatinum (CDDP). CDDP (0.1 mg/day bolus) was injected intraperitoneally to the DDN mice in which 3 X 10(7) MM2 tumor cells were inoculated the day before treatment. The anticancer effect was examined from 2nd to 7th consecutive day after tumor cells implantation, and the following results were obtained. 1) MM2 tumor cells were covered by powder-like material consisted with CDDP and were destroyed. 2) Mesothelial cells were also degenerated by powder-like material of CDDP. 3) In CDDP group, only a little intraperitoneal effusion and adhesion were observed. 4) These results suggested that CDDP exerted anti-tumor cell effects on peritonitis carcinomatosa.