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Updated: Mar 8, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
Hypermethylation of antisense long noncoding RNAs in acute lymphoblastic leukemia
Gerald Arthur1,2, Md Almamun3, Kristen Taylor1
1Department of Pathology & Anatomical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA.
Aim:
Long noncoding RNAs serve critical regulatory functions highly specific for a tissue and its developmental stage. Antisense long ncRNA (AS-lncRNA) methylation changes in acute lymphoblastic leukemia (ALL) versus normal pre-B-cell lymphoblasts were evaluated to identify potential differential methylation in this group of genes.
Materials & Methods:
The methylome of ALL and normal lymphoblasts was examined by the methylated CpG island recovery assay followed by NGS.
Conclusion:
The potential effect of trans regulation by AS-lncRNA through DNA/RNA binding is significant as sequence alignment analysis of the 25 most differentially methylated AS-lncRNAs revealed 368 genes containing highly similar sequences with a median nucleotide identity of 90.8% and binding span of 122 base pairs. Regulation of biological processes and anatomical structure development were over represented. ALL classification schemes based on AS-lncRNA methylation can provide new insights into its pathogenesis and treatment.
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