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Published on: February 5, 2014
Development of Diversity Arrays Technology markers as a tool for rapid genomic assessment in Nile tilapia,
C E Lind1, A Kilian2, J A H Benzie1,3
1WorldFish, Jalan Batu Maung, 11960, Bayan Lepas, Penang, Malaysia.
Abstract:
The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype-by-sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain-specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs.
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