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Targeted Multiresidue Analysis of Veterinary Drugs in Milk-Based Powders Using Liquid Chromatography-Tandem Mass
James B Wittenberg1, Kelli A Simon1, Jon W Wong1
1Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration , 5001 Campus Drive, HFS-717, College Park, Maryland 20740-3835, United States.
Abstract:
An analytical method was developed and validated for the determination of 40 veterinary drugs in various milk-based powders. The method involves acetonitrile/water extraction, solid-phase filtration for lipid removal in fat-containing matrices, and analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The limits of quantitation (LOQ) ranged from 0.02 to 82 ng/g. Acceptable recoveries (70-120%, RSD < 20%) were reached for 40 of 52 target compounds at three fortification levels in nonfat milk powder. Similar results were obtained for whole milk powder, milk protein concentrate, whey protein concentrate, and whey protein isolate. This new method will allow for better monitoring of a wide range of veterinary drugs in milk-based powders.
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