Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

2.2K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.2K
Global Regulatory Systems01:28

Global Regulatory Systems

812
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
812
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.9K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

11.0K
11.0K
Riboswitches01:56

Riboswitches

9.9K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.9K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

1.5K
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NMR of Fully and Partially <sup>13</sup>C-Enriched Biomass Enhances Pendent Group Structural Characterization.

Analytical chemistry·2026
Same author

Assembly and Reactions of Artificial Metalloenzymes in <i>Streptomyces albus</i>.

Journal of the American Chemical Society·2026
Same author

Developing carbon assimilation methods in duckweed for insights into photosynthesis and growth mechanisms.

Plant physiology·2026
Same author

The relationship between idiopathic atrial fibrillation and cancer.

Journal of osteopathic medicine·2026
Same author

TgZFP9-TgSBP3 and TgDOF5.4-TgPDH Regulatory Networks Drive Photosynthate Allocation to Oil in <i>Torreya grandis</i> Kernels.

Journal of agricultural and food chemistry·2026
Same author

TridentSynth: a webtool for the retrosynthesis of molecules using chimeric type I polyketide synthases and chemoenzymatic pathways.

Nucleic acids research·2026

Related Experiment Video

Updated: Mar 8, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.7K

Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants.

Yan Liang1,2, Sarah Richardson1,2, Jingwei Yan1,2

  • 1Joint BioEnergy Institute, EmeryStation East, 5885 Hollis Street, 4th Floor, Emeryville, California 94608, United States.

ACS Synthetic Biology
|January 18, 2017
PubMed
Summary

Scientists engineered a novel two-component system for precise control of gene expression in plants. This genetic engineering tool enables robust transgene repression and fine-tuning of metabolic pathways for improved crop development.

Keywords:
CRISPR/Cas9Csy4endoribonucleasegene regulationsynthetic biologytwo-component repressor system

More Related Videos

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

10.2K
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

51.2K

Related Experiment Videos

Last Updated: Mar 8, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.7K
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

10.2K
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

51.2K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Plant Biotechnology

Background:

  • Precise regulation of gene expression is crucial for genetic engineering and developing advanced regulatory circuits.
  • Fine-tuning metabolic pathways is essential to prevent the accumulation of undesirable intermediate compounds.
  • Existing methods for transgene expression control present challenges in achieving tight regulation.

Purpose of the Study:

  • To develop a novel two-component expression-repression system for tight control of transgene product synthesis.
  • To demonstrate the functionality and effectiveness of this system in both monocotyledonous and dicotyledonous plant species.
  • To explore the potential diversity and applications of bacterial endoribonuclease-based regulatory systems in plants.

Main Methods:

  • Utilized the endoribonuclease Csy4 and its specific recognition sequence from Pseudomonas aeruginosa.
  • Manipulated the 5' untranslated region (5'UTR) of messenger RNA (mRNA) to create a two-component system.
  • Employed bioinformatics to identify orthologous systems and validated their activity in planta.

Main Results:

  • Developed a functional two-component system for transgene expression control in plants.
  • Achieved >400-fold repression of transgene expression and synchronized repression.
  • Demonstrated tissue-specific and stimuli-dependent transgene repression capabilities.
  • Identified 54 orthologous systems and validated several in planta, highlighting system diversity.

Conclusions:

  • The developed Csy4-based two-component system offers robust and versatile control over transgene expression in plants.
  • This system facilitates precise regulation of transgene products, supporting metabolic engineering and synthetic biology applications.
  • The identified diversity of bacterial systems suggests broad potential for developing novel plant gene regulation tools.