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Published on: April 20, 2017
Photoinduced Cross-Linking of Short Furan-Modified DNA on Surfaces
Cinthya Véliz Montes1,2, Henry Memczak3, Ellen Gyssels4
1Department of Analytical Chemistry; Reference Materials, BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Str. 11, D-12489 Berlin, Germany.
Abstract:
We report for the first time the formation of site-specific interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen. 1O2, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.

