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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Mar 8, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Development of Fully Automated Low-Cost Immunoassay System for Research Applications.

Guochun Wang¹, Champak Das¹, Bradley Ledden¹

¹1 SFC Fluidics, Inc., Fayetteville, AR, USA.

SLAS Technology

|January 18, 2017

Summary

A new low-cost, automated immunoassay system using capillary columns offers rapid, accurate protein analysis for small research labs. This portable device significantly reduces assay time and running costs, making advanced diagnostics more accessible.

Keywords:

ELISA diagnostic tools immunoassay system lab automation microfluidics microtechnology

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Last Updated: Mar 8, 2026

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Published on: September 10, 2017

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Published on: February 23, 2020

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Published on: November 13, 2021

Area of Science:

Biomedical Engineering
Analytical Chemistry
Laboratory Automation

Background:

Enzyme-linked immunosorbent assay (ELISA) automation is highly desirable for small research settings.
Existing automated systems can be expensive and complex for routine use in smaller labs.

Purpose of the Study:

To design, develop, and evaluate a portable, fully automated, low-cost immunoassay system.
To demonstrate the system's adaptability for various protein analytes and its potential for routine operation.

Main Methods:

The system utilizes disposable capillary columns as reaction sites.
Real-time calibration is employed to enhance assay accuracy.
The design focuses on automation, reduced material use, and ease of adaptation for new targets.

Main Results:

The system successfully performs assays in under 75 minutes.
Demonstrated performance with rabbit immunoglobulin G (IgG) and interleukin 6 (IL6) assays.
Achieved extremely low running costs due to automation and reduced material consumption.

Conclusions:

The developed portable automated immunoassay system is suitable for small research environments.
The system offers a cost-effective and efficient solution for protein analysis.
Further optimization could extend its applicability to broader diagnostic testing scenarios.