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Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7
Liang Xu1,2, Mohamed Farah Eisa Ahmed3, Lancine Sangare4
1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, 1 Nongda South Road, Xibeiwang Town, Haidian District, Beijing 100193, China. xuliang19824@163.com.
A novel enzyme from Bacillus shackletonii effectively degrades aflatoxins in food and feed. This discovery offers a promising biological solution for aflatoxin detoxification, improving food safety and reducing economic losses.
Area of Science:
- Food Science
- Microbiology
- Biotechnology
Background:
- Aflatoxin (AF)B₁ contamination in food and feed poses significant economic and health risks.
- Microbial degradation of aflatoxins presents a viable preventive strategy.
Purpose of the Study:
- To identify bacterial isolates with aflatoxin-degrading activity.
- To characterize the enzyme responsible for aflatoxin detoxification.
Main Methods:
- Screening of 43 bacterial isolates for AFB₁-reducing activity.
- Identification of isolate L7 as Bacillus shackletonii.
- Purification and characterization of the Bacillus aflatoxin-degrading enzyme (BADE).
Main Results:
- Bacillus shackletonii isolate L7 demonstrated high degradation rates for AFB₁, AFB₂, and AFM₁.
- A thermostable enzyme, BADE, was purified with a molecular mass of 22 kDa.
- BADE exhibited optimal activity at 70 °C and pH 8.0, with specific cofactor requirements.
Conclusions:
- Bacillus shackletonii harbors a potent aflatoxin-degrading enzyme (BADE).
- BADE shows significant potential for application in the detoxification of aflatoxins during food and feed processing.
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