Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Transplantation01:24

Tissue Transplantation

1.1K
Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
1.1K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

85.4K
Overview
85.4K
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

2.1K
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
2.1K
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

616
Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living...
616
Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

615
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
615
Kidney Transplant III: Nursing Management01:16

Kidney Transplant III: Nursing Management

472
Postoperative Nursing Management for Kidney Transplant PatientsPostoperative nursing management care includes monitoring the surgical site, encouraging early movement, and promoting lung health through breathing exercises. Nurses also administer prescribed medications like H2-blockers, such as famotidine, or proton pump inhibitors, like omeprazole, to help prevent gastrointestinal ulcers and bleeding. Fungal infections in the mouth and bladder can result from immunosuppressive and antibiotic...
472

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Donor bone marrow together with recipient regulatory T cells induces chimerism without irradiation in kidney transplantation.

Science translational medicine·2026
Same author

Effect of felzartamab on the molecular phenotype of antibody-mediated rejection in kidney transplant biopsies.

Nature medicine·2025
Same author

A Randomized Trial Comparing Imlifidase to Plasmapheresis in Kidney Transplant Recipients With Antibody-Mediated Rejection.

Clinical transplantation·2024
Same author

The Clinical Relevance of the Infiltrating Immune Cell Composition in Kidney Transplant Rejection.

Journal of the American Society of Nephrology : JASN·2024
Same author

Epithelial cell states associated with kidney and allograft injury.

Nature reviews. Nephrology·2024
Same author

Letter to the Editor Re: Letter by Naesens et al. Microvascular inflammation: Gene expression changes do not necessarily reflect pathogenesis.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2022

Related Experiment Video

Updated: Mar 8, 2026

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
06:48

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Published on: July 25, 2014

13.9K

Comprehensive Analysis of Transcript Changes Associated With Allograft Rejection: Combining Universal and Selective

P F Halloran1,2, J M Venner1, K S Famulski1,3

  • 1Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada.

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|January 20, 2017
PubMed
Summary

Identifying kidney transplant rejection involves analyzing gene expression. New algorithms reveal transcripts associated with rejection, distinguishing between T cell-mediated and antibody-mediated types for better diagnosis.

Keywords:
basic (laboratory) research/sciencebiopsykidney transplantation/nephrologyrejectionrejection: T cell mediated (TCMR)rejection: antibody-mediated (ABMR)

More Related Videos

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach
09:15

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Published on: May 7, 2019

17.2K
Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research
09:36

Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

Published on: April 26, 2019

6.2K

Related Experiment Videos

Last Updated: Mar 8, 2026

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
06:48

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Published on: July 25, 2014

13.9K
Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach
09:15

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Published on: May 7, 2019

17.2K
Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research
09:36

Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

Published on: April 26, 2019

6.2K

Area of Science:

  • Nephrology
  • Immunology
  • Bioinformatics

Background:

  • Kidney transplant rejection poses a significant challenge in transplantation.
  • Accurate molecular markers are needed for early and specific diagnosis of rejection types.

Purpose of the Study:

  • To identify and annotate top gene transcripts associated with kidney transplant rejection.
  • To differentiate between T cell-mediated rejection (TCMR) and antibody-mediated rejection (ABMR) using molecular signatures.

Main Methods:

  • Utilized eight class-comparison algorithms on microarray data from 703 kidney biopsies.
  • Employed both universal (all rejection vs. non-rejection) and selective (specific rejection types vs. others) comparison strategies.
  • Integrated top transcripts from universal and selective algorithms for unsupervised analysis using principal component analysis (PCA).

Main Results:

  • Universal algorithms identified interferon-γ inducible transcripts.
  • Selective algorithms were necessary to distinguish between ABMR and TCMR, with TCMR showing a stronger signal.
  • Highly associated rejection transcripts were not entirely specific, with some elevated in non-rejection biopsies due to shared innate immunity pathways.
  • PCA analysis using a combined set of transcripts effectively separated rejection from non-rejection (PC1) and distinguished TCMR from ABMR (PC2).

Conclusions:

  • Accurate classification of kidney transplant rejection requires a diverse case mix and precise histologic diagnosis.
  • Both selective and universal transcriptomic algorithms are crucial for a comprehensive understanding of rejection-associated molecular changes.
  • Molecular profiling, including C4d-negative ABMR, aids in distinguishing rejection subtypes and improving diagnostic accuracy.