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Updated: Mar 8, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Janus PEGylated gold nanoparticles: a robust colorimetric probe for sensing nitrite ions in complex samples
Yanmei Xiong1, Mingmin Li1, Huiqiao Liu1
1State Key Laboratory of Medicinal Chemical Biology, Research Center for Analytical Sciences, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, College of Chemistry, Nankai University, Tianjin 300071, China. yangjie035@nankai.edu.cn liudb@nankai.edu.cn.
We developed a novel Janus PEGylated gold nanoparticle (AuNP) probe for detecting nitrite (NO2-). This probe offers enhanced stability, specificity, and sensitivity for analyzing complex samples.
Area of Science:
- Nanotechnology
- Analytical Chemistry
- Biomedical Engineering
Background:
- Gold nanoparticles (AuNPs) are widely used in sensing applications.
- Developing stable and sensitive probes for complex sample analysis remains a challenge.
Purpose of the Study:
- To design and synthesize a Janus PEGylated AuNP probe with asymmetric functionalization.
- To evaluate the probe's performance in the sensitive and specific determination of nitrite.
Main Methods:
- Asymmetric functionalization of AuNPs with polyethylene glycol (PEG) and 4-aminobenzenethiol (4-ABT).
- Characterization of the Janus PEGylated AuNP probe.
- Assessing probe stability, signal robustness, specificity, and sensitivity for nitrite detection.
Main Results:
- The Janus PEGylated AuNP probe exhibited high colloidal stability.
- The probe demonstrated robust signals, high specificity, and sufficient sensitivity for nitrite determination.
- Successful application in various complex sample matrices.
Conclusions:
- The asymmetric functionalization strategy is effective for creating advanced AuNP probes.
- The developed Janus PEGylated AuNP probe is a promising tool for sensitive and specific nitrite detection.
